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Doctors are looking for faster and more efficient systems for the diagnosis of infectious diseases in order to start treatment at the earliest for better patient management. One example of an infectious disease is tuberculosis where early treatment is critical to prevent:

The spread of diseases in the community
The emergence of multi drug resistant tuberculosis

Keeping this concern of doctors in mind, our laboratory has introduced BACTEC 460 TB, which is a rapid system for automated detection, susceptibility testing and differentiation on mycobacterium.

Though there are myriads of diagnostic tools available for the diagnosis of TB, culture is always considered as a "Gold Standard". The biggest draw back for the conventional LJ culture method is the time factor. It takes approximately 5-6 weeks just to know whether the sample is positive or negative. The above disadvantage has been overcome by the BACTEC 460 TB system in laboratories, which saves practically 30-50 days when compared with the LJ culture.

BACTEC 460 has not only increased the rapidity and sensitivity of the culture system but has also introduced very reliable and standardized methods for differentiation and drug sensitivity, which is very important in the proper management of the patient.

Advantages of BACTEC

Features
Benefits
  Better Recovery   For Authentic and accurate result interpretation
  Sensitivity Recovery   Save cost, suffering and help to prevent MDR-TB's
  Rapid Recovery   For Better patient care through early therapy

Comparison of Various Diagnostic Methods in DIAGNOSIS OF MYCOBACTERIA

Parameters
ZN
Staining
LJ
Culture
BECTEC 460 Culture
PCR
(Polymerase Chain Reaction)
TMA
(Transcription mediated Amplification)
  Time
30 Minutes
Avg. 3-6 Weeks
Avg. 4-3 Days
Avg. 1-2 Days
4 Hrs
  Sensitivity
<40%
55-60%
70-80%
98-99%
98-99%
  Drug Susceptibility
-ve
+ve
+ve
-ve
-ve
  Differentiation
-ve
+ve
+ve
-ve
-ve
  Cost
Cheap
Cheap
Moderate
Expensive
Expensive
  Detection of live Bacteria only
-
-
+
-
+

Bibliography

 
Venkataraman P, Herbert D, Paramasivan C.N. Evaluation of the BACTEC radiometric method in the early diagnosis of tuberculosis. I.J.M.Res 1998; 108: 120-127
 
Margie A, Morgan, Carl D. Comparison of radiometric method (BACTEC) and conventional culture media for recovery of mycobacteria from smear negative specimens. JCM 1983; 18(2): 384
 
Glenn D. Roberts, Norman L. Goodman, etal. Evaluation of BACTEC radiometric method for recovery of mycobacterium tuberculosis from Acid-Fast Smear -Positive Specimens. JCM 1983; 18 (3): 689.4. Casal M. Laboratory approaches to my cobacerial susceptibility to antibiotics. Rev Exp Quimioterap 1995; 8: 184-9

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