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| Info for Cervical Cancer Diagnosis |
| Info for Genital Herpes Diagnosis |
| Info for PCR for Tricomonas Vaginalis |
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| Rapid AFB Culture |
| a
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Doctors are looking for faster and more efficient systems for the
diagnosis of infectious diseases in order to start treatment at
the earliest for better patient management. One example of an infectious
disease is tuberculosis where early treatment is critical to prevent:
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The spread of diseases in the community |
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The emergence of multi
drug resistant tuberculosis |
Keeping this concern of doctors in mind, our laboratory has introduced
BACTEC 460 TB, which is a rapid system for automated detection,
susceptibility testing and differentiation on mycobacterium.
Though there are myriads of diagnostic tools available for the diagnosis
of TB, culture is always considered as a "Gold Standard".
The biggest draw back for the conventional LJ culture method is
the time factor. It takes approximately 5-6 weeks just to know whether
the sample is positive or negative. The above disadvantage has been
overcome by the BACTEC 460 TB system in laboratories, which saves
practically 30-50 days when compared with the LJ culture.
BACTEC 460 has not only increased the rapidity and sensitivity of
the culture system but has also introduced very reliable and standardized
methods for differentiation and drug sensitivity, which is very
important in the proper management of the patient.
Advantages of BACTEC
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Benefits |
| Better
Recovery |
For
Authentic and accurate result interpretation |
| Sensitivity
Recovery |
Save cost, suffering
and help to prevent MDR-TB's |
| Rapid
Recovery |
For
Better patient care through early therapy |
Comparison of Various Diagnostic Methods
in DIAGNOSIS OF MYCOBACTERIA
Parameters |
ZN
Staining |
LJ
Culture |
BECTEC
460 Culture |
PCR
(Polymerase Chain Reaction) |
TMA
(Transcription mediated Amplification) |
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30
Minutes |
Avg.
3-6 Weeks |
Avg.
4-3 Days |
Avg.
1-2 Days |
4
Hrs |
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<40% |
55-60% |
70-80% |
98-99% |
98-99% |
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-ve |
+ve |
+ve |
-ve |
-ve |
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-ve |
+ve |
+ve |
-ve |
-ve |
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Cheap |
Cheap |
Moderate |
Expensive |
Expensive |
Detection
of live Bacteria only
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- |
- |
+ |
- |
+ |
Bibliography
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Venkataraman
P, Herbert D, Paramasivan C.N. Evaluation of the BACTEC
radiometric method in the early diagnosis of tuberculosis.
I.J.M.Res 1998; 108: 120-127 |
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Margie
A, Morgan, Carl D. Comparison of radiometric method (BACTEC)
and conventional culture media for recovery of mycobacteria
from smear negative specimens. JCM 1983; 18(2): 384 |
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Glenn
D. Roberts, Norman L. Goodman, etal. Evaluation of BACTEC
radiometric method for recovery of mycobacterium tuberculosis
from Acid-Fast Smear -Positive Specimens. JCM 1983; 18 (3):
689.4. Casal M. Laboratory approaches to my cobacerial susceptibility
to antibiotics. Rev Exp Quimioterap 1995; 8: 184-9 |
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